Darya Chervyakova

Personal information

e-mail darya.dcd@gmail.com
Work address

Laboratory of the cell nucleus structure and dynamics, Biology Research Institute, Saint-Petersburg State University, St. Petersburg, 14th Line of Vasilyevsky Island, 29, liter A, 199178, Russia.


Education & Qualifications

2013 - p.t.
Current position: post-doctoral fellow at the Cytology and Histology Department, Biology Research Institute, Saint-Petersburg State University, Russia.
1998 - 2013
Researcher at the Molecular and Radiation Biophysics Department of Petersburg Nuclear Physics Institute, the National Research Centre Kurchatov Institute, Russia.
2011
Participation in the TIP49a protein structural research project at the Institut Laue-Langevin, Grenoble, France.
2009

PhD degree in molecular biology awarded at the Institute of Cytology, RAS, Saint-Petersburg (thesis: The specific properties of the highly recombinogenic chimeric proteins RecAX21 and RecAX53 (Pseudomonas aeruginosa/Escherichia coli)).

2001

One-year research fellow at the National Cancer Institute, the National Institutes of Health, USA.The role of the CDC25 A phosphatase in S phase checkpoint in study of the human cells.

1998

Master of Sciences degree in Biophysics (Department of Biophysics, The Faculty of Physics and Mechanics, Saint-Petersburg State Polytechnical University).

1997
2-months practice at the Hans-Knoll Institute, Jena, Germany. Study of the molecular cloning and protein-protein interactions via two-hybrid yeast systems.

Major Publications

  1. Kulikova T., Chervyakova D., Zlotina A., KrasikovaA., Gaginskaya E.
    Giant poly (A)-rich RNP aggregates form at terminal regions of avian lampbrush chromosomes. Chromosoma, 2015. 1-16.
    DOI:10.1007/s00412-015-0563-4
  2. Trofimova I., Chervyakova D., Krasikova A.
    Transcription of subtelomere tandemly repetitive DNA in chicken embryogenesis. Chromosome Research. 2015.
    DOI: 10.1007/s10577-015-9487-3.
  3. Shvetsov AV, Lebedev DV, Chervyakova D., Bakhlanova IV, Yung IA, Radulescu A, Kuklin AI, Baitin DM, Isaev-Ivanov VV.
    Structure of the RecX protein compex with the presynaptic RecA filament: molecular dynamics simulations and small angle neutron scattering. 2014.FEBS Letters.
  4. Chervyakova D., Lanzov V.
    Hyperrecombinogenity of the chimeric protein RecAX53 (Esherichia coli/Pseudomonas aeruginosa) is caused by its increased dynamics. Ecological genetics. 2008. 4: 47-54. (The article in Russian)
  5. Baitin D., Bakhlanova I., Chervyakova D., Kil Y., Lanzov V., Cox M.
    Two RecA protein types that mediate different modes of hyperrecombination. Journal of Bacteriology. 2008. Apr. 190(8):3036-45.
  6. Goloudina A., Yamaguchi H., Chervyakova D., Appella E., Fornace A.J. Jr, Bulavin D.
    Regulation of human Cdc25A stability by Serine 75 phosphorylation is not sufficient to activate a S phase checkpoint. Cell Cycle. 2003. Sep-Oct. 2(5):473-8.
  7. Chervyakova D., Kagansky A., Petukhov M., Lanzov V.
    [L29M] substitution in the interface of subunit-subunit interactions enhances Escherichia coli RecA protein properties important for its recombinogenic activity. Journal of Molecular Biology. 2001. Dec. 7. 314(4):923-35.

 

Patent:Patent  2011138475 from 20.09.2011 «The way of globotriose obtaining”. Authors: Bobrov KS, Bobrikova DR, Borisova AS, Kulminskaya AA, Rychkov GN, Chervyakova DB, Shabalin KA, Eneyskaya EV. Registered 01.11.2013 by the Federal Service for Intellectual Property of Russia (Rospatent), Moskow, Russia. (In Russian)


Working knowledge

Molecular biology: cloning, site-directed mutagenesis, northern-blotting; protein-protein interactions study via two-hybrid yeast system.

Biochemistry: native and tagged recombinant protein purification, ATPase, kinase and phosphatase activity study, western blotting, IP. Preparation of large amounts of untagged purified proteins for structural research by small-angle neutron scattering (RecA, RecX, TIP49a).

Cell culture methods: mouse embryonal fibroblast obtaining; human, rat, mouse cell lines cultivation; transfection.