darya.dcd@gmail.com | |
Work address | Laboratory of the cell nucleus structure and dynamics, Biology Research Institute, Saint-Petersburg State University, St. Petersburg, 14th Line of Vasilyevsky Island, 29, liter A, 199178, Russia. |
2013 - 2016 |
Post-doctoral fellow at the Cytology and Histology Department, Biology Research Institute, Saint-Petersburg State University, Russia. |
1998 - 2013 |
Researcher at the Molecular and Radiation Biophysics Department of Petersburg Nuclear Physics Institute, the National Research Centre Kurchatov Institute, Russia. |
2011 |
Participation in the TIP49a protein structural research project at the Institut Laue-Langevin, Grenoble, France. |
2009 |
PhD degree in molecular biology awarded at the Institute of Cytology, RAS, Saint-Petersburg (thesis: The specific properties of the highly recombinogenic chimeric proteins RecAX21 and RecAX53 (Pseudomonas aeruginosa/Escherichia coli)). |
2001 |
One-year research fellow at the National Cancer Institute, the National Institutes of Health, USA.The role of the CDC25 A phosphatase in S phase checkpoint in study of the human cells. |
1998 |
Master of Sciences degree in Biophysics (Department of Biophysics, The Faculty of Physics and Mechanics, Saint-Petersburg State Polytechnical University). |
1997 |
2-months practice at the Hans-Knoll Institute, Jena, Germany. Study of the molecular cloning and protein-protein interactions via two-hybrid yeast systems. |
Patent:Patent № 2011138475 from 20.09.2011 «The way of globotriose obtaining”. Authors: Bobrov KS, Bobrikova DR, Borisova AS, Kulminskaya AA, Rychkov GN, Chervyakova DB, Shabalin KA, Eneyskaya EV. Registered 01.11.2013 by the Federal Service for Intellectual Property of Russia (Rospatent), Moskow, Russia. (In Russian)
Molecular biology: cloning, site-directed mutagenesis, northern-blotting; protein-protein interactions study via two-hybrid yeast system.
Biochemistry: native and tagged recombinant protein purification, ATPase, kinase and phosphatase activity study, western blotting, IP. Preparation of large amounts of untagged purified proteins for structural research by small-angle neutron scattering (RecA, RecX, TIP49a).
Cell culture methods: mouse embryonal fibroblast obtaining; human, rat, mouse cell lines cultivation; transfection.